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. 2017 Jun 13;91(4):613–630. doi: 10.1111/tpj.13591

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© 2017 The Authors. The Plant Journal published by John Wiley & Sons Ltd and Society for Experimental Biology.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Substitution of Pro residues in Pro‐rich domain affects abundance and localization of F4C.

(a) Three independent homozygous transformant lines of FLA4:F4C_P1234A and FLA4:F4C are compared on a western blot.

(b) F4C line #4 and F4C:P1234A #5 are compared with identical imaging settings with normal (top panel) and increased contrast (bottom panel).

(c) An XZ‐section shows F4C‐P1234A derived signal in cell corners (arrowheads).

(d) F4C_P1234A co‐localizes with endosomal markers RabA1e‐cerulean and RabF2a‐cerulean while there is only weak overlap with erRFP.

(e) Affinity‐purified F4C_P1234A reacts with LM14 but not with JIM13. Labelling with the complex‐N‐glycan reacting HRP antibody shows a strong relative increase of small fragments compared to a size reduced (arrowhead) full‐length fragment.