Figure 3.

Effect of mutation of the MtrC or OmcA multiheme protein (strains ΔmtrC and ΔomcA, respectively) on the KIE values. The KIE was estimated as the reciprocal of the ratio of I_c before and after the addition of D₂O. The I_c was monitored before and after the addition of D₂O at concentrations up to 4 % (v/v) in systems containing WT, ΔmtrC, or ΔomcA cells in the presence 2 μm riboflavin (RF; a) or flavin mononucleotide (FMN; b). The data are presented as the mean±standard error of the mean (n>3). The inset panels and figure show the KIE data obtained in the absence of flavins with the same range of D₂O concentrations and a schematic illustration of EET by the RF‐bound OM Cyt c complex.