Table 1.

Study designs

Experiment	Dosing schedule	Dosage (mg/kg per week)	Dosage (mg/kg per time)	Treated days after the first immunization (efficacy) or Treated days (Toxicity)	Number of mice	Observations
1–1	Control	0	0	8–14	12	Arthritis score (Figure 1)
	Once weekly	7	7	8	12
	Thrice weekly	7	2.33	8 (twice daily), 9	12
	Once daily (1.0 mg/kg)	7	1	8–14	12
1–2	Control	0	0	1–21	6	Arthritis score (Figure 2)
	Once weekly	7	7	1, 8, 15	6
	Thrice weekly	7	2.33	1 (twice daily), 2 8 (twice daily), 9 15 (twice daily), 16	6
	Once daily (1.0 mg/kg)	7	1	1–21	6
2	Control	0	0	1–21	18	Arthritis score (Figure 3)
	Once weekly	7	7	1, 8, 15	6
	Thrice weekly	7	2.33	1 (twice daily), 2 8 (twice daily), 9 15 (twice daily), 16	18
	Once daily (0.1 mg/kg)	0.7	0.1	1–21	6
	Once daily (0.25 mg/kg)	1.75	0.25	1–21	6
	Once daily (0.5 mg/kg)	3.5	0.5	1–21	6
	Once daily (1.0 mg/kg)	7	7	1–21	6
3	Normal	0	0	1–21	6	Type II collagen antibody (Figure 4)
	CIA	0	0	1–21	6
	Thrice weekly	7	2.33	1 (twice daily), 2 8 (twice daily), 9 15 (twice daily), 16	6
	Once daily (0.25 mg/kg)	1.75	0.25	1–21	6
	Once daily (1.0 mg/kg)	7	7	1–21	6
4	Control	0	0	0–20	6	WBC counts (Figure 5) ALT and BUN levels (Table 2)
	Thrice weekly	7	2.33	0–20	6
	Once daily (0.25 mg/kg)	1.75	0.25	0 (twice daily), 1 7 (twice daily), 8 14 (twice daily), 15	6
	Once daily (1.0 mg/kg)	7	7	0–20	6