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. 2001 Jun 15;29(12):e57. doi: 10.1093/nar/29.12.e57

Figure 3.

Figure 3

Specific amplification of guanylated subgenomic and genomic HAV RNA. (A) According to the scheme on top, subgenomic in vitro transcripts (lanes 1–3), RNA of HAV-infected cells (lanes 4–6) and mock-infected cells (lanes 7–9) were subjected to the PAP reaction with GTP and subsequently to the HAV-specific amplification. RT–PCR was performed either with oligo(dT12–18) or oligo(dC9T6) as antisense primers. The sense primer for PCR was primer #2 in all cases. (B) RT–PCR products derived from cell extracts containing HAV RNA rescued after transfection of transcripts rHAV-A0, rHAV-A14, rHAV-A20 and rHAV-A26. The PCR product of 432 bp is marked by arrows. DNA standards are indicated on the left (M).