Table 1. Poly(A) tail length of viral RNA obtained after transfection of in vitro transcripts.

HAV in vitro transcript used for transfectiona	Number of adenosine residues in the poly(A) tail of rescued viral RNAb
rHAV-A0	ndc
rHAV-A14	24 – 29e
rHAV-A20	54 – 60e
rHAV-A26	26 – 37e,f

^aThe name of the RNA indicates the number of adenosine residues in the cDNA.

^bNumber of adenosine residues of multiple clones derived from PCR products.

^cNot detected (HAV-specific ELISA and RT–PCR was negative).

^eCell extracts were ELISA and RT–PCR positive.

^fDue to second site mutations, rHAV-A₂₆ replicates less efficiently and possibly poly(A) elongation is retarded.