Table 1.
Comparisons of tumor vessel structure and function.
| 1. Collagen VI Null (Brain) | 2. Tks5 Knockdown (Mammary) | 3. NG2 Null (Mammary) | 4. Mac-NG2ko (Brain) | |
|---|---|---|---|---|
| decreased tumor growth | 2-fold | 4-fold | 2-fold | 6-fold |
| decreased vessel density | no change | no change | no change | no change |
| decreased PC/EC sheathing | no change | ND | 1.6-fold | 2.5-fold |
| decreased basement membrane | 2-fold | ND | 1.5-fold | 8-fold |
| decreased PC maturation | 2-fold | ND | 2.1-fold | 5-fold |
| decreased EC sprouting | 2-fold | ND | 2.3-fold | 3-fold |
| decreased vessel diameter | 1.5-fold | 3-fold | 1.4-fold | 2.3-fold |
| increased vessel leakage | 3-fold | 2.4-fold | 3.4-fold | 5-fold |
| decreased vessel patency | 1.4-fold | ND | ND | 2-fold |
| increased tumor hypoxia | 7-fold | 3.3-fold | 2.3-fold | 12-fold |
| increased total VEGF-A | 1.3-fold | ND | 1.5-fold | 3-fold |
| decreased vessel VEGF-A | 2-fold | 3-fold | ND | 3-fold |
| increased diffuse VEGF-A | 2-fold | ND | 2-fold | 4-fold |
Vascular parameters were compared in 4 different tumor models. (1) Germline collagen VI ablation in host stroma of intracranial B16F10 melanomas. (2) Tks5 knockdown in MDA-MB-231 mammary tumors. (3) Germline NG2 ablation in host stroma of MMTV-PyMT mammary tumors. (4) Myeloid-specific ablation of NG2 in host stroma of intracranial B16F10 melanomas. Changes in each parameter are expressed as fold change in tumors in the experimental models compared to tumors in control mice. PC = pericyte. EC = endothelial cell. PC/EC = pericyte ensheathment of endothelial cells. ND = not determined.