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In Vitro Osteogenic Differentiation:(a) Alizarin Red staining showed that BMMSCs at 20% and 50 % confluence can not show in vitro osteogenic differentiation potential (A, B). 70, 80 and 100% confluences were similar in osteogenic differentiation in vitro (C, D, E). (b) % of total area stained with Alizarin Red dye, calcium deposition mg/well ALP activity (μg/mg protein) for osteogenic differentiated 20, 50, 70, 80 and 100% confluent BMMSCs.
