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. Author manuscript; available in PMC: 2018 Jul 5.
Published in final edited form as: Biochemistry. 2017 Jun 21;56(26):3403–3413. doi: 10.1021/acs.biochem.7b00266

Figure 8.

Figure 8

DSC results for fully reconstituted thin filaments of (A) wild-type, (B) Tm D230N, and (C) cTnT R92L variants. The fwhm of the actin–Tm binding interaction is represented by the sharp peak near 47 °C and represents the cooperativity of the interaction. This interaction is outlined in the wild-type data with a dashed line. The wild-type complex peak (0.993 ± 0.01 °C) is wider than that of the peak from the Tm D230N complex (0.883 ± 0.01 °C) but narrower than that of the cTnT R92L complex (1.38 ± 0.14 °C). The standard deviation resulted from two or three independent experiments.