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. Author manuscript; available in PMC: 2017 Aug 30.
Published in final edited form as: Neuroimage. 2009 Nov 10;49(4):3122–3131. doi: 10.1016/j.neuroimage.2009.11.005

Fig. 1.

Fig. 1

Experimental procedure, ROI definition, and MRI in the sham model. (a) The middle cerebral artery was occluded for 60 minutes for the three MCAO groups. During the MCAO or sham operation, diffusion-weighted MRI and MR angiography were acquired to check whether the occlusion was successful. MnCl2 solution was administered immediately after MCA reperfusion for the day 1 groups, 10 days after reperfusion for the day 11 groups, and 21 days after the reperfusion for the day 22 groups. MRI measurements were performed exactly 24 hours after the MnCl2 administration for all groups. Finally, perfusion fixation and brain extraction were executed for immunohistochemical staining. (b) The ischemic core was determined as the small ellipse having major and minor axes half the length of the fitted ellipse (red region), and the ischemic periphery was determined as the region remaining after removing the ischemic core from the fitted ellipse (blue region). (c) Typical T1-weighted manganese-enhanced MRI (MEMRI), (d) T2-, and (e) diffusion-weighted MRI. No unilateral signal change was observed for any of the sham groups.