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. Author manuscript; available in PMC: 2018 Feb 27.
Published in final edited form as: Dev Cell. 2017 Feb 27;40(4):381–391.e3. doi: 10.1016/j.devcel.2017.02.002

Figure 3. Increased eye progenitor incorporation does not contribute to eye-specific regeneration.

Figure 3

(A) BrdU immunofluorescence (IF) together with FISH for opsin. Scale bar, 10 μm.

(B–E), BrdU/opsin double-positive PRNs per animal or per eye after surgeries indicated in text or cartoons. (B) BrdU delivery Day 2, animal fixation Day 8. Decapitated but not eye-resected animals had significantly more BrdU+ PRNs than uninjured animals. (C) BrdU delivery-fixation intervals denoted on x-axis as day of delivery, day of fixation. Asterisks indicate significant increase above uninjured condition for that interval. Day 2–8 interval data is also shown in (B). PRN incorporation is higher in eye-resected animals than uninjured controls for Day 0–6 (p=0.0235) and Day 1–7 (p=0.0120) intervals by Student’s t-test. n≥5 animals per condition. (D) BrdU delivery Day 1, fixation Day 7. n=7 animals per condition. (E) BrdU delivery Day 1, fixation Day 7. n=7 animals. See also Figure S2. Data are represented as mean ± SD. Dots represent values from individual animals. In (B) and (C), statistical significance was assessed by one-way ANOVA comparing eye-resected and decapitated animals to uninjured animals for same interval, in (D) by Student’s t-test, and in (E) by paired Student’s t-test (**p<0.01, ***p<0.001); NS, not significant.

(F) SMEDWI IF with FISH for opsin and DAPI labeling, 5 days after indicated surgeries. Scale bar, 10 μm.

(G) SMEDWI/opsin double-positive PRNs per animal 5 days after indicated surgeries. n=8 animals per condition.