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. Author manuscript; available in PMC: 2018 Jul 18.
Published in final edited form as: Biochemistry. 2017 Jul 7;56(28):3579–3586. doi: 10.1021/acs.biochem.7b00138

Figure 2.

Figure 2

a) HTTex1Q46 seeds accelerate the fibrillization of HTTex1Q46 and HTTex1Q25. Tht fluorescence assay of 25 μM HTTex1Q46 and HTTex1Q25 in the presence of 0.2%, 1%, and 5% HTTex1Q46 fibril seeds. Protein aggregation is initiated by the addition of 1 unit/280 μg EKMax. HTTex1Q46 and HTTex1Q25 without seeds and HTTex1Q46 without seeds and without EKMax served as controls. Only 5% of seeds led to significant Tht fluorescence for HTTex1Q25. Fluorescence intensities of curves showing a significant increase were baseline corrected and normalized to maximum intensity. Controls that did not show an increase in fluorescence were normalized according to HTTex1Q46 without seeds b) HTTex1Q25 seeds can seed the fibrillization of HTTex1Q46. Tht fluorescence assay of 25 μM HTTex1Q46 in the presence of 0.2%, 1%, and 5% of HTTex1Q25 fibril seeds. HTTex1Q46 without seeds served as control. c) Average of the Tht kinetics half times calculated from 3 repeats of the Tht time courses shown in a) and b).