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. Author manuscript; available in PMC: 2018 Jul 18.
Published in final edited form as: Biochemistry. 2017 Jul 7;56(28):3579–3586. doi: 10.1021/acs.biochem.7b00138

Figure 3.

Figure 3

The aggregation of HTTex1Q25 is incomplete 4.5 days after seeding. The normalized EPR intensities of HTTex1Q25 and HTTex1Q46 spin labeled at residue 35 (i.e. within the polyQ domain) are shown immediately after adding 1 unit/280 μg EKMax in the presence of 5% preformed HTTex1Q46 seeds and after 4.5 days. The change in intensity reflects the amount of monomer remaining in solution (see Figure S3). Where the HTTex1Q46 signal descended to about a tenth of its original intensity and a significantly broadened line indicated complete aggregation, the HTTex1Q25 signal showed only a modest decline in intensity and no change in lineshape indicating that the majority of the protein stayed monomeric even after 4.5 days of aggregation.