Fig. 8.
miR-574-5p overexpression suppresses BACE1 expression and restores synaptic impairment following PM2.5 exposure. (a) Significant changes in miR-574-5p expression were detected via qPCR. (b1) Representative protein bands of immunoblot analysis for BACE1 and RFP. (b2) Quantification of BACE1 and RFP expression. (c1) Representative protein bands of immunoblot analysis for AMPA (GluA1 and GLuA2), NMDA (GluN1, GRIN2A, and GRIN2B) and RFP. (c2) Quantification of AMPA (GluA1 and GLuA2), NMDA (GluN1, GRIN2A, and GRIN2B) and RFP expression. (d1) Representative protein bands of immunoblot analysis for PSD-95 and RFP. (d2) Quantification of PSD-95 and RFP expression. The data are expressed as the means ± SE (n = 6 to 8 mice/group). (e1) Time courses of changes in the field excitatory postsynaptic potential (fEPSP) slope under different treatments. (e2) Mean values of the fEPSP slope averaged from 56 to 60 min after theta burst stimulation (TBS). The data are expressed as the means ± SE (n = 6 mice/group). */# P < 0.05; **/## P < 0.01. LV-Con + Veh = LV-scramble control + vehicle; LV-574 + Veh = LV-miR-574-5p + vehicle; LV-Con + PM2.5 = LV-scramble control + PM2.5; LV-574 + PM2.5 = LV-miR-574-5p + PM2.5