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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Crit Rev Biochem Mol Biol. 2016 Dec 23;52(1):96–106. doi: 10.1080/10409238.2016.1269717

Figure 2. Perturbations in growth conditions and/or stress responses that decrease rRNA synthesis result in the disappearance of RNA Pol foci and changes in nucleoid organization.

Figure 2

Shown are SIM images of DNA, RNA Pol (RNAP) and an overlay of RNAP (green) and DNA (red) from a fast-growing cell (LB, 37°C) as a control, and after different perturbations induced by SHX treatment for 30 min (+SHX 30′), nutrient down shift from LB to M63+Glu for 5 min (>M63 5′) and after high salt shock (0.5 M NaCl) for 10 min (+NaCl 10′) and 20 min (+NaCl 20′). The cell shape is outlined by a dotted line. The scale bar represents 1 μm.