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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Crit Rev Biochem Mol Biol. 2016 Dec 23;52(1):96–106. doi: 10.1080/10409238.2016.1269717

Figure 3. Formation and compartmentalization of RNA Pol foci at the surface of the compact nucleoid require multiple copies of rRNA operons located either in the genome or on an extrachromosomal plasmid.

Figure 3

Shown are SIM images of DNA, RNA Pol (RNAP) and an overlay of RNAP (green) and DNA (red) in Δ6rrn and Δ6rrn/prrnB cells either under optimal growth conditions (LB, 37°C) (first and third panels) or after SHX treatment for 30 min (+SHX) (second and fourth panels). Note that the defect of the Δ6rrn strain in the formation of RNA foci and nucleoid structure can be complemented by a plasmid-borne rRNA operon (prrnB) in the cell (compare the first and third panels). The cell shape is outlined by a dotted line. The scale bar represents 1 μm.