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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Crit Rev Biochem Mol Biol. 2016 Dec 23;52(1):96–106. doi: 10.1080/10409238.2016.1269717

Figure 4. Nascent rRNA-binding protein NusB forms foci and colocalizes with RNA Pol foci in fast-growing cells.

Figure 4

Shown are SIM images of DNA, RNA Pol (RNAP), NusB, two-color overlays of RNAP (green) and NusB (red), NusB(red) and DNA(green), and three-color overlays of RNAP(green), NusB (red) and DNA (blue) from a representative fast-growing E. coli cell (LB, 37°C). Note that NusB foci are at the periphery of the nucleoid (separate red and green colors on the RNAP/NusB overlay) and the NusB signals perfectly colocalize with RNAP signals (overall yellow color on the RNAP/NusB overlay). The cell shape is outlined by a dotted line. The scale bar represents 1 μm.