Shown are SIM images of DNA, RNA Pol (RNAP), NusB, two-color overlays of RNAP (green) and NusB (red), NusB(red) and DNA(green), and three-color overlays of RNAP(green), NusB (red) and DNA (blue) from a representative fast-growing E. coli cell (LB, 37°C). Note that NusB foci are at the periphery of the nucleoid (separate red and green colors on the RNAP/NusB overlay) and the NusB signals perfectly colocalize with RNAP signals (overall yellow color on the RNAP/NusB overlay). The cell shape is outlined by a dotted line. The scale bar represents 1 μm.