Shown are SIM images of DNA, RNA Pol (RNAP), SSB, two-color overlays of RNAP (green) and SSB (red), SSB(red) and DNA(green), and three-color overlays of RNAP(green), SSB (red) and DNA (blue) from a representative fast-growing E. coli cell (LB, 37°C). Note that SSB foci and RNA Pol foci are largely located at different positions (red and green colors on the overlay of SSB and RNAP). Note also that most of the SSB foci appear to be separated from high intensities of DNA signals in the nucleoids (red and green colors on the overlay of SSB and DNA). The cell shape is outlined by a dotted line. The scale bar represents 1 μm.