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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Crit Rev Biochem Mol Biol. 2016 Dec 23;52(1):96–106. doi: 10.1080/10409238.2016.1269717

Figure 5. Spatial segregation of transcription foci and replisomes tracked by SSB in fast-growing cells.

Figure 5

Shown are SIM images of DNA, RNA Pol (RNAP), SSB, two-color overlays of RNAP (green) and SSB (red), SSB(red) and DNA(green), and three-color overlays of RNAP(green), SSB (red) and DNA (blue) from a representative fast-growing E. coli cell (LB, 37°C). Note that SSB foci and RNA Pol foci are largely located at different positions (red and green colors on the overlay of SSB and RNAP). Note also that most of the SSB foci appear to be separated from high intensities of DNA signals in the nucleoids (red and green colors on the overlay of SSB and DNA). The cell shape is outlined by a dotted line. The scale bar represents 1 μm.