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. 2017 Aug 29;15:69. doi: 10.1186/s12958-017-0289-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Expression and subcellular localization of Plk1 in porcine oocytes during the MI-to-MII transition. (a) Expression of Plk1 was examined using western blot analysis. Plk1 was expressed in porcine oocytes during the MI-to-MII transition, and a relatively higher Plk1 protein level was detected in MI compared to ATI and MII stages; * P < 0.05; ** P < 0.01. (b) Subcellular localization of Plk1 in porcine oocytes using immunofluorescent staining. Plk1 was enriched at spindle pole regions at MI and MII stages and closely overlapped with α-tubulin leading to the barrel-shaped spindle. Plk1 was associated with the spindle midzone region at TI stage. Red, Plk1; green, spindle; blue, chromosome. Scale bar, 20 μm