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. 2017 Aug 29;15:69. doi: 10.1186/s12958-017-0289-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effect of GSK461364 treatment during MI-to-MII stage on pbI extrusion of porcine oocytes. (a) Most of the control oocytes extruded pbI and finished meiotic maturation while a significantly larger proportion of the GSK461364 treated oocytes failed to extrude pbI. Scale bar, 20 μm. (b) The rate of extruding pbI was decreased in a dose-dependent manner in GSK461364-treated groups. (c) GSK461364 treatment significantly eliminated the phosphorylation of Plk1 in porcine oocytes. ** P < 0.01; *** P < 0.001