Figure 9.

Biased (Amp3) and unbiased (Amp4) products from the NlaIII digest of the amplified linker-DiTag molecules. NlaIII digests were resolved on two separate gels as described in Figure 8. Shown here is one of four lanes from two separate DiTag amplifications from the same DiTag ligation. Note the similarity of both lanes and that this gel cannot reliably detect a GC content bias. The image length of the gel for Amp3 was reduced by 34% to line up the bands with similar molecular weights on the different gels. No other modifications were made to these images.