Table 1. Summary of DiTag amplifications.

DiTag amplification	Average GC contenta (%)	Method usedb
Amp1	48.3	RT
Amp2	58.4	RT
Amp3	55.8	RT
Amp4	49.7	Cold
Amp5	48.5	Cold

The rows in bold highlight DiTag amplifications affected with a GC content bias.

^aCalculated as described in Materials and Methods.

^bRefers to whether the phenol extraction after the NlaIII digest to release free DiTags was performed at room temperature (RT) or on ice and centrifuged at 4°C (Cold).