Fig. 6.

Downstream process validation using a phagocytosis assay. a A. pallida cells were labeled with LysoTracker Deep Red and sorted to LysoTracker high and low expression. b–c The sorted cells were co-incubated overnight with green fluorescent beads for analysis of phagocytosis. b Example of the bead positive cells gated in the LysoTracker low (left panel) and high (right panel) populations. c Analysis of bead positive cells of three replicates of the sorted populations. The bead positive cells are approximately three - fold higher in the LysoTracker high population. ANOVA P* < 0.05