Table 1.
Screened compounds to differentiate coral cells
| Compound Name | Company and Catalog | Fluorescent Indicator | Label | Differ. Cells (Symb.+) | Conc. |
|---|---|---|---|---|---|
| 3-dodecanoyl-NBD Cholesterol | CC: 13220 | Cholesterol uptake | N | N (N) | 1 μM |
| MeOSuc-Ala-Ala-Pro-Val-AMC | CC: 14907 | Elastase activity | N | N (N) | 1 μM |
| Dihydroethidium | CC: 12013 | Intracellular superoxide | Y | Y (Y) | 1 μM |
| Pyronin Y | CC: 14488 | dsRNA | Y | Y (Y) | 0.2 μM |
| ThioFluor623 | CC: 13083 | Intracellular thiols | Y | Y (Y) | 1 μM |
| Rhodamine Phenylglyoxal | CC: 16172 | Protein citrullination | Y | Y (Y) | 0.2 μM |
| CAY10455 | CC: 10005072 | Arachidonoyl ethanolamide uptake | Y | Y (N) | 0.2 μM |
| Dibenzylfluorescein | CC: 16808 | Cytochrome P450 activity | Y | N (Y) | 0.2 μM |
| BCECF-Acetoxymethyl ester | CC: 15922 | Intracellular pH | Y | Y (Y) | 0.2 μM |
| NBD-FTY720 phenoxy HCl | CC: 16841 | Sphingosine-1-phosphate | Y | N (N) | 1 μM |
| 10-Acetyl-3,7-dihydroxyphenoxazine | CC: 10010469 | Hydrogen Peroxide/Peroxidase | Y | N (N) | 1 μM |
| 2-NBDG | CC: 11046 | Glucose uptake | Y | N (N) | 1 μM |
| 1-NBD-decanoyl-2-decanoyl-sn-Glycerol | CC: 9000341 | Diacylglycerol uptake | Y | N (N) | 1 μM |
| (Z-Ala-Ala-Ala-Ala)2Rh110 | CC: 11675 | Elastase activity | N | N (N) | 1 μM |
| 5-Cyano-2,3-di-(p-tolyl)tetrazolium Cl | CC: 15926 | Aerobic metabolic activity | N | N (N) | 1 μM |
| Coumarin Boronic Acid pinacolate ester | CC: 10818 | Peroxynitrite | N | N (N) | 1 μM |
| Garcinol | CC: 10566 | Histone acetyltransferase inhibitor | N | N (N) | 1 μM |
| WSP-1 | CC: 11179 | Hydrogen Sulfide | N | N (N) | 1 μM |
| Zinpyr-1 | CC: 15122 | Intracellular Zinc ion | Y | N (Y) | 0.2 μM |
| DPPP | CC: 62237 | Hydroperoxides | N | N (N) | 1 μM |
| Acridine Orange | CC: 14338 | DNA dye | Y | Y (Y) | 0.2 μM |
| Fura-PE3-Acetoxymethyl ester | CC: 15366 | Intracellular Calcium | Y | Y (N) | 0.2 μM |
| Lucigenin | CC: 14872 | Intracellular Chloride ion | Y | N (Y) | 1 μM |
| CONA-Lectin | TFS: C21402 | Binds α-man, α-glc | Y | Y (N) | 2 μg/ml |
| EUA-Lectin | SA: L8262 | Binds α-L-fuc and (glcNAc)2 | Y | N (N) | 2 μg/ml |
| PNA-Lectin | SA: L7381 | Binds β-gal(1 → 3)galNAc | Y | Y (Y) | 2 μg/ml |
| WGA-Lectin | TFS: W32465 | Binds sialic acid and Nac | Y | Y (N) | 2 μg/ml |
| pHrodo Red | TFS: P35372 | Intracellular pH indicator | Y | Y (lables) | 5 μM |
| LysoTracker Deep Red | TFS: L12492 | Acidic organelles | Y | Y (lables) | 0.2 μM |
| CellROX green | TFS: C10492 | Reactive oxygen species | Y | Y (lables) | 1 μM |
| Phen Green | TFS: P6763 | Heavy metal indicator | Y | Y (lables) | 1 μM |
| ALDAFLUOR | ST: 1700 | ALDH-bright cells | Y | Y (lables) | 0.4 μg/ml |
| DAPI | TFS: D1306 | AT regions of DNA | Y | Y (Y) | 3 μM |
| PI | TFS: R37108 | DNA | Y | Y (N) | 5 μg/ml |
P. damicornis cell suspensions were incubated with indicated chemical compounds and analyzed by FACS. Companies used: CC Cayman Chemical, TFS Thermo Fisher Scientific, SA Sigma-Aldrich, ST Stemcell Technologies. If the chemical compound is fluorescently labeling cells it is indicated as Y (Yes) and if the result is the same as the control it is indicated as N (No) in the label column. If the labeling can clearly differentiate cellular populations by the level of labeling it is indicated as Y in the differentiation column of cells, differentiation capability of cells that are positive to Symbiodinium indicated in scopes. The concentration used throughout the study are indicated in the last column