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. Author manuscript; available in PMC: 2017 Aug 30.
Published in final edited form as: ACS Chem Biol. 2017 Apr 27;12(6):1556–1565. doi: 10.1021/acschembio.7b00143

Figure 6.

Figure 6

PmC11 Cleavage specificity of human proteins. (A) Addition of active PmC11 to HT-29 lysate generates new N-termini that are labeled with a biotin tag via subtiligase treatment.44–46 Streptavidin enrichment and subsequent LC-MS/MS peptide identification provide the proteins and cleavage sequences that are susceptible to PmC11 proteolysis (magenta N-termini generated from PmC11 activity). (B) Sequence logo of the 56 peptides identified to be targeted by PmC11 in HT-29 lysates correlates with the sequence specificity determined by MSP-MS (Figure 2B) and the crystal structure (Figure 5B).