Table 2.
Effects of 6-azauridine and cidofovir combinations on yield of vaccinia (WR strain) virus from Vero 76 cells.
Cidofovir (μM) | ||||||
---|---|---|---|---|---|---|
6-Azauridine (μM) | 0 | 8 | 16 | 32 | 64 | 128 |
32 | 5.76 ± 0.25 | 3.88 ± 0.88a,b | 3.50 ± 0.88 | 2.62 ± 0.35 | 2.38 ± 0.05 | 2.11 ± 0.21 |
16 | 6.88 ± 0.69 | 4.91 ± 0.62 | 4.28 ± 0.09 | 2.76 ± 0.51 | 2.46 ± 0.28 | 2.13 ± 0.04 |
8 | 6.94 ± 0.20 | 5.85 ± 0.02 | 5.13 ± 0.31 | 3.87 ± 0.03 | 3.05 ± 0.42 | 2.31 ± 0.29 |
4 | 7.08 ± 0.84 | 6.26 ± 0.42 | 5.66 ± 0.32 | 4.64 ± 0.44 | 3.37 ± 0.13 | 2.31 ± 0.07 |
2 | 7.65 ± 0.54 | 6.82 ± 0.27 | 6.54 ± 0.07 | 5.73 ± 0.02 | 4.02 ± 0.01 | 2.70 ± 0.34 |
0 | 7.66 ± 0.15 | 7.11 ± 0.27 | 6.70 ± 0.20 | 6.08 ± 0.01 | 4.52 ± 0.38 | 3.81 ± 0.50 |
Mean value ± SD (Log10 PFU/mL), calculated from 2 independent virus yield reduction assays, using three wells in each assay evaluated separately for virus titer determinations.). The infectious virus dose for inoculation was approximately 240 PFU per culture in each assay.
The shading indicates that the combination is synergistic at the concentrations used, as determined by a two-dimensional method (Schinazi et al., 1982).