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. Author manuscript; available in PMC: 2017 Aug 30.
Published in final edited form as: J Mol Biol. 2015 Sep 5;427(22):3587–3597. doi: 10.1016/j.jmb.2015.09.001

Figure 3. 15N-1H HSQC spectra of uniformly labeled 15N RAM.

Figure 3

(a) Spectrum of 15N RAM at 800 MHz. Most resonances are reasonably well dispersed for an IDP, facilitating assignment with standard triple resonance experiments. (b) Overlay of 15N RAM spectra with (red) and without (black) a 1.4:1 molar excess of CSL at 600 MHz. Resonances that move upon RAM:CSL binding are labeled and correspond to residues at the RAM N-terminus. Unassigned resonances that move are labeled with an asterisk. Conditions: 25 mM sodium phosphate pH 6.5, 50 mM sodium chloride, 0.1 mM TCEP, and 1 mM EDTA at 20 °C.