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. Author manuscript; available in PMC: 2017 Aug 30.
Published in final edited form as: ACS Chem Neurosci. 2016 Jun 17;7(8):1166–1173. doi: 10.1021/acschemneuro.6b00127

Figure 3.

Figure 3

Biotinylated photoaffinity probes. (a) Chemical structure of the biotinylated probes. (b, c) Biotinylated probes and clickable probes (20 nM) were incubated with HeLa membranes in the presence or absence of 1 μM competitor BMS-163 and UV-irradiated, followed by click chemistry with biotin azide for clickable probes only, pull-down with streptavidin resin, and Western blot analysis for PS1–NTF. (d) The solubilized γ-secretase was captured under native conditions (0.25% CHAPSO) by 163-BP3–biotin and 163-BP3–L-biotin. The bound complex was eluted and analyzed by Western blot analysis.