Fig. 5.

Laminin-332 promotes directional melanocyte migration. (A) Phase-contrast micrographs of melanocytes cultured in the absence of exogenous extracellular matrix substrates (No ECM), on collagen I (Col I) or laminin-332 substrate (Lam 332). Confluent cultures were wound-scraped, and phase-contrast images were obtained at the indicated times following wounding. The broken lines represent the width of the scrape-wound at time=0. Scale bars: 250 µm. (B) Cell-free surface areas of scrape-wounds remaining at the indicated times post-wounding were assessed using ImageJ. The results show the mean wound surface area values±s.d. (n=5 different cell isolates; two technical replicates per cell isolate), and have been normalized to the original scrape-wound area, which is set to 100%. *P<0.05 relative to cells plated without exogenous ECM at the corresponding time following wounding (two-way ANOVA).