Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 22;6(8):1229–1234. doi: 10.1242/bio.026294

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 3. — Regulation of larval protein synthesis by dMyc. (A) The hsflp-out system was used to induce ubiquitous UAS-dMyc expression in third instar larvae. Control larvae expressed UAS-GFP alone. 24 h following transgene induction, larvae were inverted and incubated in Schneider's media+puromycin (5 µg/ml) for 40 min. Equal amounts of whole larval protein extracts were then analyzed by western blotting. Left, western blot with anti-puromycin antibody. Right, Ponceau S staining showing total protein levels. Genotypes: control=ywhsflp¹²²/+; +/+; act>CD2>GAL4, UAS-GFP/+, dMyc=ywhsflp¹²²/+; UAS-dMyc/+; act>CD2>GAL4, UAS-GFP/+. (B) UAS-dMyc clones were generated in larval fat body cells using the flp-out system. Larvae were inverted and incubated in Schneider's media+puromycin (5 µg/ml) for 40 min. Tissues were then immunostained with and anti-puromycin antibody. The nuclear GFP-marked cells overexpressing UAS-dMyc (arrows) show increased puromycin incorporation compared to surrounding non-GFP marked wild-type cells (arrowheads). Genotype: =ywhsflp¹²²/+; UAS-dMyc/+; act>CD2>GAL4, UAS-GFP/+. (C) UAS-GFP clones were generated in larval fat body cells using the flp-out system. Larvae were inverted and incubated in Schneider's media+puromycin (5 µg/ml) for 40 min. Tissues were then immunostained with an anti-puromycin antibody. The GFP-marked cells overexpressing (arrows) show no change in puromycin incorporation compared to surrounding non-GFP marked wild-type cells (arrowheads). Genotype: ywhsflp¹²²/+; +/+; act>CD2>GAL4, UAS-GFP/+.