Table 2. Comparison of TFIIIB subunits of S.pombe, S.cerevisiae and humana.
S.pombe TFIIIB | S.cerevisiae homolog | Human homolog | . | S.pombe cosmid no. | |||||||||
Subunit |
Mass (kDa) |
Length (amino acids) |
Subunit |
Length |
Identity (%) |
Similarity (%) |
Alignmentb |
Subunit |
Length |
Identity (%) |
Similarity (%) |
Alignmentb |
EMBL locusc |
spTBP | 25 | 231 | scTBP | 240 | 93 | 95 | 179 | hTBP | 339 | 80 | 90 | 179 | SPAC30.12 |
spBrf | 56 | 500 | scBrf | 596 | 37 | 52 | 601 | hBRF | 675 | 47 | 64 | 294 | SPBC13E7 |
spB″ | 58 | 507 | scB″ | 594 | 34 | 57 | 138 | hB″ | 1388 | 35 | 55 | 148 | SPCC1919.14c |
aComparisons were conducted using the BLAST 2 Sequences program at NCBI with default parameters. The percent identity and percent similarity over the region of greatest match was recorded.
bThe number of positions in the alignment, including gaps.
cCosmid no./EMBL locus is accessible at http://www.sanger.ac.uk/Projects/S_pombe/FUNCAT/trna_syn.shtml.