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. Author manuscript; available in PMC: 2017 Aug 30.
Published in final edited form as: Gene Ther. 2003 Sep;10(18):1608–1615. doi: 10.1038/sj.gt.3302053

Figure 1.

Figure 1

Gene transfer by electroporation in the explanted lung. A quantity of 20 μg of pCMV-lux-DTS in 200 μl of 10 mM Tris, pH 8, containing 1 mM EDTA and 140 mM NaCl was injected into the trachea of euthanized mice. Immediately following DNA delivery, eight square wave pulses of 10 ms duration each were administered at a field strength of 200 V/cm via Tweezertrode electrodes placed on either side of individual lobes, which had been exposed by opening the chest. Lungs received no DNA (control), DNA without electroporation (DNA only), or DNA and electroporation (n=4 per condition). Following DNA delivery, the lungs were placed in DMEM containing 10% FBS and antibiotics for 24 h, at which time luciferase expression was measured. Mean expression levels were calculated and standard error of the mean (s.e.m) was determined using Instat software (GraphPad Inc., San Diego, CA, USA).