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A cartoon of a hypothetical tripartite synapse. Here each piece, glia, presynaptic cell, and postsynaptic cell are all expressing a different form of receptor, which is labeled with an orthogonal photoswitchable ligand (PORTLs and PTLs). The synaptic cleft also has a freely diffusing PCL. Distinctly different absorption spectra for each photoswitchable ligand would allow exact spatiotemporal control at the subcellular level.
