Extended Data Figure 3. ERF is a negative regulator of androgen signalling.
Related to Fig. 2. a, Mouse prostate organoids infected with non-targeting shRNA (shNT) or targeting ERF (shErf_m), grown in three-dimensional culture. For RT–qPCR, n = 2 biological replicates. For gel source data, see Supplementary Fig. 1. b, RT–qPCR analysis of the Pten+/+ organoids; n = 2 biological replicates. c, RNA-seq analysis of organoids derived from Pten+/+ and Pten−/− mouse prostates infected with non-targeting shNT or shErf_m; n = 2 biological replicates. d, Pten+/+ organoid RNA-seq (n = 2 biological replicates) interrogated by GSEA for expression signature of Witte basal prostate cancer18. e, Same data interrogated by GSEA for Nelson androgen up expression signature20.