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. 2017 Sep 1;26(17):1236–1246. doi: 10.1089/scd.2017.0123

FIG. 4.

FIG. 4.

SLC7A11 overexpression is correlated with increased tumorsphere formation. (A) Photomicrographs of SLC7A11-modified U251 and control cells after 10 days of culture in CSC-enriching or normal media, scale bars, 300 and 100 μm, respectively. (B) SLC7A11-modified U251 and control cells were cultured for a total of 12 days in CSC-enriching media. Numbers of tumorspheres formed at indicated time points were calculated. Data shown are means from a representative experiment. (C) Limiting dilution analysis of frequencies of sphere-forming cells present after 14 days of culture for SLC7A11-modified U251 and control cells. (D) SLC7A11-modified U251 and control cells were cultured for a total of 12 days in CSC-enriching media. Number of tumorspheres was counted before single-cell dissociation and replating for another 12 days of enrichment in CSC-enriching media for secondary tumorsphere cultures and again for tertiary cell cultures. Data shown are mean ± SD from a representative experiment. **P < 0.01; ***P < 0.001. CSC, cancer stem cell.