Fig. 4.

Assessment of cytotoxic effects of select agents on HUVECs (A, B) and iPSC-derived ECs (C, D) after 24-h chemical treatment with (A, C) and without (B, D) cytokine exposure. Reproducibility of concentration–response plots for histamine (blue) and chloroquine (red) for the three cytotoxic endpoints each represented by the different shapes. Concentration–response graphs shown were prepared in GraphPad using a normalized nonlinear fit. Each line represents the mean ± SEM for a single experiment with three replicates.