Table 4.

Assay Quality Control—Angiogenesis Assays

		Interplate reproducibilitya		Interday reproducibilitya		%CV of negative controlsb			Z′-factorb
Metric	Cells	Gelc	Hydrod	Gel	Hydro	Gel	Hydro	Chemicals	Gel	Hydro
Total tube length	HUVEC	S: 0.70 (<0.0001)	S: 0.81 (<0.0001)	S: 0.72 (<0.0001)	S: 0.75 (<0.0001)	12	6.6	Nocoe	0.35	0.67
		P: 0.66 (<0.0001)	P: 0.88 (<0.0001)	P: 0.86 (<0.0001)	P: 0.86 (<0.0001)			Suraminf	−0.46	−2.8
	iPSC-Endog	S: 0.61 (<0.0001)	S: 0.35 (0.0024)	S: 0.55 (<0.0001)	S: 0.37 (0.0016)	15	28	Noco	−1.5	−0.31
		P: 0.70 (<0.0001)	P: 0.56 (<0.0001)	P: 0.63 (<0.0001)	P: 0.59 (<0.0001)			Suramin	0.49	−5.7
Mean tube length	HUVEC	S: 0.82 (<0.0001)	S: 0.64 (<0.0001)	S: 0.80 (<0.0001)	S: 0.57 (<0.0001)	6.1	2.7	Noco	−0.011	−0.66
		P: 0.80 (<0.0001)	P: 0.80 (<0.0001)	P: 0.84 (<0.0001)	P: 0.75 (<0.0001)			Suramin	−1.5	−2.0
	iPSC-Endog	S: 0.72 (<0.0001)	S: 0.40 (0.0004)	S: 0.75 (<0.0001)	S: 0.65 (<0.0001)	14	4.3	Noco	−7.0	−0.58
		P: 0.84 (<0.0001)	P: 0.61 (<0.0001)	P: 0.83 (<0.0001)	P: 0.76 (<0.0001)			Suramin	−3.4	−77
Total tube area	HUVEC	S: 0.56 (<0.0001)	S: 0.81 (<0.0001)	S: 0.70 (<0.0001)	S: 0.71 (<0.0001)	14	7.1	Noco	0.44	0.65
		P: 0.54 (<0.0001)	P: 0.86 (<0.0001)	P: 0.83 (<0.0001)	P: 0.80 (<0.0001)			Suramin	−0.35	−3.9
	iPSC-Endog	S: 0.61 (<0.0001)	S: 0.38 (0.001)	S: 0.53 (<0.0001)	S: 0.56 (<0.0001)	15	31	Noco	−1.9	−0.42
		P: 0.71 (<0.0001)	P: 0.55 (<0.0001)	P: 0.63 (<0.0001)	P: 0.71 (<0.0001)			Suramin	0.51	−7.0

 ^aS: Spearman's ρ, P: Pearson's r. Correlation analysis sample size ranged from n = 60–72. P values provided below correlation values.

 ^bSample size ranges from n = 18–36.

 ^cGel: Geltrex™.

 ^dHydro: SP-105 hydrogel.

 ^eNoco: 100 nM nocodazole.

 ^fSuramin: 50 μM suramin.

 ^giPSC-Endo: iCell endothelial cells from Cellular Dynamics International.

 Plots of the data used to calculate Z′-factors shown in this table are included as Supplementary Figure S2.