Table 5.

Concentration Response Analysis—Angiogenesis Assay

			Average POD values ± SDa
Metric	Cells	Chemical	Geltrex™	Hydrogel
Total tube length	HUVECs	Nocodazole	0.028 ± 0.029	0.010 ± 0.00042
		Suramin	8.0 ± 2.6	>50
		SU5402	2.9 ± 3.9	0.063 ± 0.0046
		TAB	14 ± 17	0.38 ± 0.34
	iPSC-Endob	Nocodazole	0.0018 ± 0.0024	0.030 ± 0.029
		Suramin	2.8 ± 2.2	>50
		SU5402	0.057 ± 0.061	0.64 ± 0.57
		TAB	1.0 ± 0.94	8.1 ± 9.5
Mean tube length	HUVECs	Nocodazole	0.036 ± 0.0048	0.017 ± 0.014
		Suramin	12 ± 3.2	>50
		SU5402	9.4 ± 10	>50
		TAB	10 ± 2.7	0.17 ± 0.045
	iPSC-Endob	Nocodazole	0.064 ± 0.10	0.032 ± 0.036
		Suramin	9.0 ± 4.7	>50
		SU5402	2.5 ± 2.7	42 ± 11
		TAB	10 ± 1.7	20 ± 20
Total tube area	HUVECs	Nocodazole	0.33 ± 0.53	0.010 ± 0.0002
		Suramin	7.6 ± 3.7	>50
		SU5402	0.96 ± 0.96	0.064 ± 0.0052
		TAB	13 ± 15	0.42 ± 0.35
	iPSC-Endob	Nocodazole	0.0012 ± 0.0019	0.030 ± 0.027
		Suramin	3.2 ± 2.5	>50
		SU5402	0.13 ± 0.13	0.65 ± 0.57
		TAB	7.9 ± 13	9.4 ± 11.0

 ^aValue (μM) at which the concentration–response fit curve crosses beyond 1 SD of the mean control value. Values shown represent the mean and SD of the single curve fit for three separate experiments each containing three replicates per concentration.

 ^biPSC-Endo: iCell endothelial cells from Cellular Dynamics International.

 POD, point-of-departure; SD, standard deviation; TAB, tetraoctyl ammonium bromide.