Table 6.

Assay Quality Control—Cytotoxicity Assay

		Interplate reproducibilitya		Interday reproducibilitya		%CV of negative controls		Z′-factorb (N = 12–36)
Metric	Cells	(+)c	(−)d	(+)	(−)	(+)	(−)	(+)	(−)
Nuclei content	HUVEC	S: 0.81 (<0.0001)	S: 0.72 (<0.0001)	S: 0.79 (<0.0001)	S: 0.71 (<0.0001)	6.4	6.6	0.73	0.55
		P: 0.84 (<0.0001)	P: 0.89 (<0.0001)	P: 0.88 (<0.0001)	P: 0.90 (<0.0001)
	iPSC-Endoe	S: 0.81 (<0.0001)	S: 0.78 (<0.0001)	S: 0.71 (<0.0001)	S: 0.58 (<0.0001)	14.4	7.4	−1.2	−0.19
		P: 0.83 (<0.0001)	P: 0.89 (<0.0001)	P: 0.77 (<0.0001)	P: 0.80 (<0.0001)
Viable cell staining	HUVEC	S: 0.81 (<0.0001)	S: 0.72 (<0.0001)	S: 0.81 (<0.0001)	S: 0.71 (<0.0001)	6.4	6.6	0.78	0.80
		P: 0.90 (<0.0001)	P: 0.90 (<0.0001)	P: 0.90 (<0.0001)	P: 0.91 (<0.0001)
	iPSC-Endoe	S: 0.87 (<0.0001)	S: 0.77 (<0.0001)	S: 0.82 (<0.0001)	S: 0.51 (<0.0001)	15.4	7.4	0.53	0.64
		P: 0.97 (<0.0001)	P: 0.95 (<0.0001)	P: 0.89 (<0.0001)	P: 0.78 (<0.0001)
ATP content	HUVEC	S: 0.87 (<0.0001)	S: 0.78 (<0.0001)	S: 0.80 (<0.0001)	S: 0.62 (<0.0001)	6.5	6.8	0.79	0.72
		P: 0.91 (<0.0001)	P: 0.89 (<0.0001)	P: 0.86 (<0.0001)	P: 0.91 (<0.0001)
	iPSC-Endoe	S: 0.87 (<0.0001)	S: 0.80 (<0.0001)	S: 0.80 (<0.0001)	S: 0.66 (<0.0001)	8.3	7.0	0.74	0.77
		P: 0.91 (<0.0001)	P: 0.97 (<0.0001)	P: 0.86 (<0.0001)	P: 0.93 (<0.0001)

 ^aS: Spearman's ρ, P: Pearson's r. Correlation analysis sample size ranged from n = 96. P values provided below correlation values.

 ^bTreatment with TAB at 50 μM.

 ^c(+): Cells stimulated with cytokines.

 ^d(−): Unstimulated cells.

 ^eiPSC-Endo: iCell endothelial cells from Cellular Dynamics International.

 Plots of the data used to calculate Z′-factors shown in this table are included as Supplementary Figure S3.

ATP, adenosine triphosphate.