Table 7.
Concentration Response Analysis—Cytotoxicity Assay
| Average POD values ± SDa | ||||
|---|---|---|---|---|
| Metric | Cells | Chemical | (+)b | (−)c |
| Nuclei content | HUVECs | Colchicine | 0.038 ± 0.0062 | 0.04 ± 0.001 |
| Histamine | 35 ± 45 | 75 ± 20 | ||
| Concanamycin A | 0.00073 ± 0.0009 | 0.0056 ± 0.0055 | ||
| Chloroquine phosphate | 1.0 ± 0.42 | 30 ± 24 | ||
| iPSC-Endod | Colchicine | 0.050 ± 0.014 | 0.048 ± 0.012 | |
| Histamine | 44 ± 22 | >100 | ||
| Concanamycin A | 0.0033 ± 0.0033 | 0.020 ± 0.024 | ||
| Chloroquine phosphate | 58 ± 5.0 | 17 ± 18 | ||
| Viable cell staining | HUVECs | Colchicine | 0.053 ± 0.030 | 0.039 ± 0.0005 |
| Histamine | 35 ± 46 | 76 ± 20 | ||
| Concanamycin A | 0.0009 ± 0.0001 | 0.0056 ± 0.0055 | ||
| Chloroquine phosphate | 1.2 ± 0.45 | 28 ± 23 | ||
| iPSC-Endod | Colchicine | 0.040 ± 0.0040 | 0.050 ± 0.016 | |
| Histamine | 33 ± 28 | >100 | ||
| Concanamycin A | 0.00016 ± 0.00006 | 0.017 ± 0.017 | ||
| Chloroquine phosphate | 32 ± 11 | 18 ± 19 | ||
| ATP content | HUVECs | Colchicine | 0.023 ± 0.016 | 0.044 ± 0.004 |
| Histamine | 39 ± 43 | 62 ± 28 | ||
| Concanamycin A | 0.00005 ± 0.00001 | 0.0075 ± 0.0075 | ||
| Chloroquine phosphate | 0.6 ± 0.22 | 5.3 ± 4.7 | ||
| iPSC-Endod | Colchicine | 0.035 ± 0.0038 | 0.039 ± 0.0069 | |
| Histamine | 36 ± 46 | >100 | ||
| Concanamycin A | 0.00019 ± 0.00009 | 0.0004 ± 0.00014 | ||
| Chloroquine phosphate | 9.3 ± 2.6 | 17 ± 18 | ||
aValue (μM) at which the concentration–response fit curve crosses beyond one SD of the mean control value. Values shown represent the mean and SD of the single curve fit for three separate experiments each containing three replicates per concentration.
b(+): Cells stimulated with cytokines.
c(−): Unstimulated cells.
diPSC-Endo: iCell endothelial cells from Cellular Dynamics International.