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. 2017 Sep 1;33(9):905–918. doi: 10.1089/aid.2016.0303

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© Anddre Valdivia et al., 2017; Published by Mary Ann Liebert, Inc.

This article is available under the Creative Commons License CC-BY-NC (http://creativecommons.org/licenses/by-nc/4.0). This license permits non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. Permission only needs to be obtained for commercial use and can be done via RightsLink.

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FIG. 5. — Assay of IL-17 and IL-1. (A) Baseline levels of IL-17 in plasma samples of Healthy Baseline and HIV-positive subjects with CD4+ T cell counts below 350 cells/mm³. IL-17 levels were significantly compromised in the plasma samples from HIV patients compared to the healthy individuals. IL-17 was assayed in the plasma samples by sandwich ELISA using assay kits procured from eBioscience. Data represent mean ± SE from 16 healthy individuals and 30 individuals with HIV. (B) IL-17 levels in the plasma samples from the HIV placebo group with CD4+ T cell counts below 350 cells/mm³. There was a further significant decrease in the levels of IL-17 in the plasma samples isolated from the HIV-positive subjects at 3 months post-treatment with empty liposomes. Data represent mean ± SE from 16 healthy individuals and 15 individuals with HIV placebo. (C) IL-17 levels in the plasma samples from the HIV-L-GSH group with CD4+ T cell counts below 350 cells/mm³. The levels of IL-17 stabilized in the plasma samples isolated from the HIV-positive subjects at 3 months post-treatment with L-GSH. Data represent mean ± SE from 16 healthy individuals and 15 individuals with HIV-L-GSH. (D) Baseline levels of IL-1 in plasma samples of Healthy Baseline and HIV-positive subjects with CD4+ T cell counts below 350 cells/mm³. IL-1 levels were significantly compromised in the plasma samples from HIV patients compared to the healthy individuals. IL-1 was assayed in the plasma samples by sandwich ELISA using assay kits procured from eBioscience. Data represent mean ± SE from 16 healthy individuals and 30 individuals with HIV. (E) IL-1 levels in the plasma samples from the HIV placebo group with CD4+ T cell counts below 350 cells/mm³. There was a further significant decrease in the levels of IL-1 in the plasma samples isolated from the HIV-positive subjects at 3 months post-treatment with empty liposomes. Data represent mean ± SE from 16 healthy individuals and 15 individuals with HIV placebo. (F) IL-1 levels in the plasma samples from the HIV-L-GSH group with CD4+ T cell counts below 350 cells/mm³. The levels of IL-1 stabilized in the plasma samples isolated from the HIV-positive subjects at 3 months post-treatment with L-GSH. Data represent mean ± SE from 16 healthy individuals and 15 individuals with HIV-L-GSH, *p ≤ 0.05; **p ≤ 0.01.