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. 2017 Aug 29;17:428. doi: 10.1186/s12906-017-1941-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Effects of solvent fractions of ESE on HCMV replication. HFF cells were mock-infected (lane 1) or infected with HCMV-Towne at MOI of (a) 0.1 or (b) 1 (lanes 2 to 8) and treated with DMSO (lanes 1 and 2), ESE (lane 3) or n-Hexane (lane 4), CH₂Cl₂ (lane 5), EtOAc (lane 6), n-BuOH (lane 7) or ddH₂O (lane 8) fractions of ESE at 50 μg/ml. At 3 days after infection, total DNA was harvested and the relative amounts of viral DNA were measured via qPCR using primers specific for UL123. Significant difference between samples was determined based on P values obtained from Student’s t test (* P < 0.01, ** P < 0.001)