Fig. 7.

Modifications of brain cytosolic phospholipase A₂ (cPLA₂) and neuron-specific acyl-coenzyme A synthetase 4 (nACSL4) proteins induced by arachidonic acid-enriched (ARA) diet and amyloid-β peptide 42 (Aβ₄₂). Immediately after the probe test, mice were killed, and homogenates were prepared from the cortex and hippocampus. Representative immunoblots of cortical (a and c) and hippocampal (b and d) phosphorylated cytosolic phospholipase A₂ (P-cPLA₂) and cPLA₂ (a and b) and nACSL4 (c and d) proteins from oleic acid-enriched (OLE) or ARA diet mice after NaCl or Aβ₄₂ injections are shown. Densitometric analyses were performed to determine signal intensities normalized to β-tubulin for cPLA₂ and nACSL4 and normalized to cPLA₂ for P-cPLA₂, and data are expressed as percentages of control OLE mice injected with NaCl (* p < 0.05, comparing the four groups of mice). In (a) and (b), P-cPLA₂/cPLA₂ density is shown by the black and white scales (y-axis on the left), and cPLA₂/β-tubulin is shown by the gray circles (y-axis on the right). Results are shown as mean ± SEM of immunoblots performed for all animals (OLE groups, n = 4; ARA groups, n = 6)