Table 1.
Sample name | Raw reads | Clean reads | Clean bases | Error rate (%) | Q20 (%) | Q30 (%) | GC content (%) |
---|---|---|---|---|---|---|---|
R_As24h | 50,921,692 | 48,696,660 | 7.3G | 0.02 | 96.79 | 91.95 | 50.91 |
S_As24h | 48,149,668 | 45,974,494 | 6.9G | 0.02 | 96.62 | 91.61 | 50.96 |
R_As13d | 53,198,176 | 50,759,128 | 7.61G | 0.02 | 96.49 | 91.41 | 49.43 |
S_As13d | 48,863,236 | 46,720,102 | 7.01G | 0.02 | 96.57 | 91.51 | 50.12 |
R_As19d | 51,480,496 | 49,154,934 | 7.37G | 0.02 | 96.59 | 91.57 | 50.71 |
S_As19d | 45,488,110 | 43,544,700 | 6.53G | 0.02 | 96.69 | 91.72 | 50.95 |
Note: Raw reads, sequence reads from each RNA sample; Clean reads, reads following the removal of adapters, low quality reads or reads with 'N' > 1%; Clean bases, read number times read length; Error rate, calculated error rate based on read quality; Q20 (%) and Q30 (%), percentage of bases with Phred score > 20 or 30; GC content (%), percentage of G plus C in the sequences