FIG. 2.

Neural differentiation of marmoset stem cells. (A) Schematic for NP dual-SMAD inhibition protocol using SB431542 and DMH1. Representative images throughout neural differentiation showing (B) pluripotent cell colonies, d0–8; (C) neurospheres, d8–28; (D) plated dissociated neurospheres, d30; (E) extensive process extension, d42+. The Cj-ESC (F) and Cj-iPSC (G) lines were efficiently differentiated to neurons shown by nestin (green) and βIII-tubulin (red) expression; DAPI (blue). Scale bars: (B–G), 100 μm. NP, nonpatterned.