Table 1.
Primer and probe sequences for cPCR and qPCR assays
| Target | Primer/Probes | Amplicon size (bp) | Sequence (5′–3′) | Reference |
|---|---|---|---|---|
| T. cruzi kinetoplast DNA (kDNA) | 121 (Forward) | 330 | AAATAATGTACGGG(T/G)GAGATGCATGA | [17, 24] |
| 122 (Reverse) | GGTTCGATTGGGGTTGGTGA ATATA | |||
| T. cruzi satellite DNA (SAT-DNA) | Cruzi 1 (Forward) | 165 | ASTCGGCTGATCGTTTTCGA | [18] |
| Cruzi 2 (Reverse) | AATTCCTCCAAGCAGCGGATA | |||
| Cruzi 3 (Probe) | FAM-TTGGTGTCCAGTGTGTG-NFQ-MGB | |||
| Triatomine 12S rRNA | P2B (Forward) | 163 | AAAGAATTTGGCGGTAATTTAGTCT | Present study |
| P6R (Reverse) | GCTGCACCTTGACCTGACATT | |||
| Triat (Probe) | VIC-TCAGAGGAATCTGCCCTGTA-NFQ-MGB |