Table 3.
Representation of Cq mean values for detection of the 12S rDNA gene by real-time PCR in distinct triatomine species and absence of amplification signal in animal blood samples
| Triatomine detection (1 intestine, 8–10 ng DNA) | Possible blood-meal source detection (blood, 20 ng DNA) | ||
|---|---|---|---|
| Triatomine species | Cq (Mean ± SD) | Origin | Cq (Mean ± SD) |
| Rhodnius prolixus | 25.54 ± 0.30 | Human | nd |
| Rhodnius neglectus | 26.55 ± 0.54 | Bat | nd |
| Triatoma vitticeps | 26.86 ± 1.37 | Cat | nd |
| Triatoma sordida | 24.54 ± 0.39 | Dog | nd |
| Opossum | nd | ||
| Rat | nd | ||
Abbreviations: C q threshold cycle, SD standard deviation, nd not detected