Additional file 2: Video S2. Cell adhesion behaviors on coverslips coated with ephrin-A1-Fc in stripes. HL60 cells in the VD-TNF group at a density of 3 × 105 cells/mL (1.5 mL/3.5-cm culture dish) were plated on coverslips coated with EphA2-Fc or ephrin-A1-Fc in stripes and Fc plus Matrigel overall, and placed in a 3.5 cm culture dish in an incubator (maintained at 37 °C in humidified 5% CO2/95% air; ONI-INU-F1, Tokai Hit Co., Ltd., Fujinomiya, Japan) installed on the stage of an inverted microscope (IX71, Olympus). Phase contrast images were obtained at 2 min intervals (for 16 h) using a digital camera (DP72, Olympus) controlled by software (DP2-BSW, Olympus). Videos starting from a few min after cell seeding consist of a series of time-lapse micrographs in a field wherein a region adsorbed with EphA2-Fc (Additional file 1: Video S1) or ephirn-A1-Fc (Additional file 2: Video S2) and Fc plus Matrigel (left side) and that with Fc plus Matrigel (right side) appears adjacently. (MOV 7230 kb)
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