Table 1.

Primers and cycle numbers for RT-PCR amplification of mouse mRNAs

Mouse	Primer		Product size (bp)	Annealing temp. (°C)	Cycle number
CD115	Forward	5-GGGACAGCACGAGAATATAG-3’	590	53.0	26
	Reverse	5’-CACTCTGAACTGTGTAGACG-3’
CD34	Forward	5’-ACAACCACAGACTTCCCCAA-3’	424	59.5	33
	Reverse	5’-ATTGGCCAAGACCATCAGCA-3’
EphA1	Forward	5’-TCGAGCCTTACGCCAACTAC-3’	466	69	38
	Reverse	5’-CCGATCAGCAGAGCTATTCC-3’
EphA2	Forward	5’-GAGTGTCCAGAGCATACCCT-3’	549	62.5	37
	Reverse	5’-GCGGTAGGTGACTTCGTACT-3’
EpA3	Forward	5’-TGTTGGTGCTTGTGTTGCC-3’	440	57	38
	Reverse	5’-CATTTCTTGGTGCGGATGG-3’
EphA4	Forward	5’-GGGCAGTGAATGGAGTGTCT-3’	448	61.4	38
	Reverse	5’-AGAATGACCACGAGGACCAC-3’
EphA5	Forward	5’-CTCTGGACGTGCCTTCTC-3’	319	57	38
	Reverse	5’-TCCCCAGTCCTCCAGGAA-3’
EphA6	Forward	5’-GTGCGGAGCTTGGCTATGTT-3’	373	61.4	38
	Reverse	5’-GAGCTGAAGGTGGTCTAGTG-3’
EphA7	Forward	5’-ACGGGGGAAGAAACGATGTC-3’	544	61.4	38
	Reverse	5’-GCTTCCTCAAGTGTGGCAAC-3’
EphA8	Forward	5’-TTGTGGGAGTGGAACTCGCT-3’	632	61.4	38
	Reverse	5’-CCTGTCCGTTCTGGTAATGC-3’
EphA10	Forward	5’-ACTACCTAGAAACCGAGACC-3’	493	53.0	38
	Reverse	5’-GACACCTTGTAAAACCCTGG-3’
ephrin-A1	Forward	5’-CATCATCTGCCCACATTACG-3’	463	60.0	37
	Reverse	5’-AGCAGTGGTAGGAGCAATAC-3’
ephrin-A2	Forward	5’-GGTGAGCATCAACGACTACC-3’	464	61.4	38
	Reverse	5’-CTGACACTAGGAGCCCAGAA-3’
ephrin-A3	Forward	5’-TCCGCACTACAACAGCTCAG-3’	504	62.5	38
	Reverse	5’-TAGGAGGCCAAGAGCGTCAT-3’
ephrin-A4	Forward	5’-AAGATTCAGCGCTACACACC-3’	420	61.4	38
	Reverse	5’-GATCCTCCGACTTTGCACAT-3’
ephrin-A5	Forward	5’-CGTGGAGATGTTGACGCTG-3’	585	57.0	38
	Reverse	5’-GGCTCGGCTGACTCATGTA-3’
GAPDH	Forward	5’-GACTCCACTCACGGCAAAT-3’	689	57.0	22
	Reverse	5’-TCCTCAGTGTAGCCCAAGAT-3’