Table 2.

Primers and cycle numbers for RT-PCR amplification of human mRNAs

Human	Primer		Product size (bp)	Annealing temp. (°C)	Cycle number
CD14	Forward	5’-AGAACCTTGTGAGCTGGACGAT-3’	369	61.0	24
	Reverse	5’-GAAAGTGCAAGTCCTGTGGCTT-3’
CD15	Forward	5’-TTTGGATGAACTTCGAGTCGCC-3’	360	60.0	30
	Reverse	5’-AAGCCAGGTAGAACTTGTAGCG-3’
CD16	Forward	5’-GACTGAAGATCTCCCAAAGG-3’	668	52.3	24
	Reverse	5’-CCTTCCAGTCTCTTGTTGAG-3’
CD115	Forward	5’-GTGGTAGAGAGTGCCTACTT-3’	342	53.4	30
	Reverse	5’-CCATATGACGCTTACCTCTG-3’
integrin α1	Forward	5’-GTCTATCCACGGAGAAATGG-3’	417	50.0	32
	Reverse	5’-CTCACAGAGTCCTGAAAGTC-3’
integrin α2	Forward	5’-CTACAATGTTGGTCTCCCAG-3’	440	50.0	32
	Reverse	5’-CAACATCTATGAGGGAAGGG-3’
integrin α4	Forward	5’-GATCATCTTACTGGACTGGC-3’	320	54.8	32
	Reverse	5’-CAGATCTGAGAAGCCATCTG-3’
integrin α5	Forward	5’-CCAGCCCTACATTATCAGAG-3’	390	50.0	32
	Reverse	5’-GAGATGAGGGACTGTAAACC-3’
integrin α6	Forward	5’-GGAGATAAACTCCCTGAACC-3’	416	50.0	32
	Reverse	5’-CGAGAATAGCCACTAGGATG-3’
integrin αD	Forward	5’-ATCAGCAGGCAGGAAGAATC-3’	518	50.1	32
	Reverse	5’-ACCTCGTCTTCTTCTAGCAC-3’
integrin αL	Forward	5’-GCCCTGGTTTTCAGGAATG-3’	430	53.0	32
	Reverse	5’-CCAATCCCGATGATGTAGC-3’
integrin αM	Forward	5’-GTGTGATGCTGTTCTCTACG-3’	364	50	32
	Reverse	5’-CTCCATGATTGCCTTGACTC-3’
integrin αX	Forward	5’-CCAGATCACCTTCTTGGCTAC-3’	523	61.3	32
	Reverse	5’-CTTCAGGGTGAAATCCAGCTC-3’
integrin β1	Forward	5’-TTCAAGGGCAAACGTGTGAG-3’	459	54.4	28
	Reverse	5’-CCGTGTCCCATTTGGCATTC-3’
integrin β2	Forward	5’-CAATAAACTCTCCTCCAGGG-3’	522	52.5	28
	Reverse	5’-CAGTACTGCCCGTATATCAG-3’
Rap1A	Forward	5’-GTACAAGCTAGTGGTCCTTG-3’	371	50.0	29
	Reverse	5’-CAACTACTCGCTCATCTTCC-3’
EphA1	Forward	5’-TGGTGGAACTTCCTTCGAGA-3’	446	61.2	35
	Reverse	5’-ACAATCCCAAAGCTCCACAC-3’
EphA2	Forward	5’-CGCCGGCTCTGATGCACCTT-3’	312	62.0	38
	Reverse	5’-TCCTGAGGTGCCCGGAAGAA-3’
EphA3	Forward	5’-TGCGGTCAGCATCACAACTA-3’	380	59.3	38
	Reverse	5’-TTGCTACTGCCGCTGAAATG-3’
EphA4	Forward	5’-TGCAGCTTTTGTCATCAGCC-3’	381	60.3	38
	Reverse	5’-TTAGTGACCACGCCTTCCAA-3’
EphA5	Forward	5’-CTCTGGACGTGCCTTCTC-3’	319	56.6	38
	Reverse	5’-TCCCCAGTCCTCCAGGAA-3’
EphA6	Forward	5’-TGCGAAGTCCGGGAATTTCT-3’	627	61.6	38
	Reverse	5’-ACGAACAGTGAAGGGGCATT-3’
EphA7	Forward	5’-AAAGCTGACCAAGAAGGCGA-3’	311	62.0	38
	Reverse	5’-TCAAACTGCCCCATGATGCT-3’
EphA8	Forward	5’-ATCACCTACAATGCCGTGTG-3’	341	58.4	38
	Reverse	5’-TACTCCAGGATGATGCCGTT-3’
EphA10	Forward	5’-CACGTACCAAGTGTGCAATG-3’	646	56.8	38
	Reverse	5’-CAACTGGATACCTTCGCAGA-3’
ephrin-A1	Forward	5’-GGAACCCAGACCCATAGGAG-3’	825	59.7	38
	Reverse	5’-CCCGTTTTGAGGCTGCTAGG-3’
ephrin-A2	Forward	5’-TACGTGCTGTACATGGTCAACG-3’	316	58.1	38
	Reverse	5’-GGCTGCTACACGAGTTATTGCT-3’
ephrin-A3	Forward	5’-TTTACTGCCCGCACTACAACAG-3’	523	59.7	38
	Reverse	5’-AACGTCATGAGGAAGAAGGCGA-3’
ephrin-A4	Forward	5’-GCCCCGAGACGTTTGCTTTGTA-3’	364	62.5	34
	Reverse	5’-TTGTCGGTCTGAATTGGCACCC-3’
ephrin-A5	Forward	5’-ACTCTCCAAATGGACCGCTGAA-3’	363	66.0	38
	Reverse	5’-TCAAAAGCATCGCCAGGAGGAA-3’
GAPDH	Forward	5’-GTCGGAGTCAACGGATTTGG-3’	607	57.2	21
	Reverse	5’-GGATGATGTTCTGGAGAGCC-3’