Fig. 2.

miR-222 had no effect on HCT8 cell proliferation, cell cycle and apoptosis. HCT8 cells were transfected with miR-222 mimic, mimic control, miR-222 inhibitor or inhibitor control. a Cell proliferation detected by Cell Titer 96^® A Queous Non-Radioactive Cell Proliferation Assay (MTS).Cell numbers were determined by MTS after 0, 1, 2 and 3 days. Data represent the mean ± SD of 6 independent experiments. b The cells were stained with PI and detected by FACS. The cell cycle was compared among the G0/G1 (black bar), G2/M (gray bar) and S stages (white bar). The data represent the mean ± SD, which are further detailed in Additional file 1: Table S1. c, d Apoptosis was detected by FACS. HCT8 cells were marked with Annexin V and PI, Annexin V+/PI− and Annexin V+/PI+ indicating early and late apoptosis, respectively. Data represent the mean ± SD of six independent experiments